Police allege a large number of Santa Gertrudis-cross heifers were put on the property in May.
When the owner went to collect them in September, 81 of the animals had been stolen.
Further investigations led police to arrest a 52-year-old Tungkillo man yesterday, and he was charged with theft.
He was refused bail and will appear in the Murray Bridge Magistrates Court today.
Operation Poach was introduced by SAPOL in July 2011 as a direct response to the increased reporting of livestock theft across the state.
The theft of commercial quantities of livestock has serious consequences for the South Australian primary production sector.
Operation Poach aims to bring about a sustained reduction in overall livestock theft.